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Hadwan, Mahmoud H.
- New Optimized Method for Assaying 5'-Nucletidase in Biological Fluids
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Authors
Affiliations
1 Chemistry Dept., College of Science, Babylon University, IQ
2 Surgery Dept., College of Medicine, Babylon University, IQ
1 Chemistry Dept., College of Science, Babylon University, IQ
2 Surgery Dept., College of Medicine, Babylon University, IQ
Source
International Journals of Biotechnology and Biochemistry, Vol 8, No 1 (2012), Pagination: 79-86Abstract
A simple method for assaying 5'-Nucletidase activity in biological fluids (serum, seminal plasma, spermatozoa) has been developed. This method is based on the measurement of liberated phosphate by the reaction of phosphate with molybdatein an acidic medium to produce phosphomolybdate complex, and reduce this complex to unstable molybdenum blue. Bismuth nitrate is used to stabilize phosphomolybdate complex. The results showed a good agreement with those obtained by standard method (Campbell's method) the correlation coefficient between the two methods was 0.9888 and statistical investigations of the data established very good agreement between the two set of values.Keywords
5'-Nucletidase Activity, Biological Fluids, Phosphomolybdate Complex, Bismuth Nitrate
References
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- Ismail,A. A. A., and Williams, D. G., 1974, "Scope and limitations of a kinetic assay for serum 5’-nucleotidase activity,". Clin. Chim. Acta, 55, pp.211-216.
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- Baginski, E.S., Foa, P.P., and Zak, B., 1969 "Determination of phosphate and phosphomonoesterases in biologic materials, " Am. J. Med. Technol.,35, pp.475–486.
- Cariani, L., Thomas, L., Brito., and del Castillo, J.R., 2004 "Bismuth citrate in the quantification of inorganic phosphate and its utility in the determination of membrane-bound phosphatases, " Analytical Biochemistry, 324, pp.79–83.
- Kortz, U., Sauelieff, M.G., AbouGhali, F.Y., Khalil, etal., 2002, "Heteropolymolybdates of AsIII, SeIV and TeIV functionalized by amino acids, "Angew. Chem. Int. Ed., 41, pp. 4070–4073.
- New Method for Assessment of Serum Catalase Activity
Abstract Views :238 |
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Authors
Affiliations
1 Chemistry Department, College of Science, University of Babylon, Hilla city, Babylon Governorate, P.O. 51002, IQ
1 Chemistry Department, College of Science, University of Babylon, Hilla city, Babylon Governorate, P.O. 51002, IQ
Source
Indian Journal of Science and Technology, Vol 9, No 4 (2016), Pagination:Abstract
Background: The following study presents a colorimetric method for the assessment of serum catalase activity which yields precise, accurate, reproducible results and is simplified so that clinical pathology laboratories may achieve this determination without the need for special techniques. Methods: In this method, dichromate in acetic acid is reduced to chromic acetate when heated in the presence of undecomposed hydrogen peroxide (H2O2), with the formation of perchromic acid as an unstable intermediate. Hydrogen peroxide concentration is directly proportional to the concentration of chromic acetate that produced from the reaction. The chromic acetate produced is measured calorimetrically at 570 nm. Findings: The imprecision of the method was calculated by measuring the coefficient of variation, which equals to 3.4% within run and 5.9% between run. The catalase assay performed using the kinetic method yielded a good correlation (r = 0.9771). Applications: The present method characterizes by adding a correction factor to eliminate the interference that arises from the presence of sugars, amino acids, proteins and vitamins in serum.Keywords
Catalase Activity, Clinical Pathology, New Method Serum, SpectrophotometryFull Text